Warming Kit

For warming of in vitro and in vivo embryos

Description and Prod. No.

4 vials each containing 2 ml medium in glass bottles, Prod. No. 2.21.008 Obtain higher embryo survival and pregnancy rates with vitrification than after slow freeze Particularly in vitro and biopsied embryos benefit from this cryopreservation method Pre-heat media 38.8°C prior to use -leave lid on in order to avoid evaporation

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts

The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants)

Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second

See the detailed protocol on Vitrification and Warming of embryos