For vitrification of in vitro and in vivo embryos
Description and Prod. No.
3 vials each containing 2 ml medium in glass bottles, Prod. No. 2.20.006 Obtain higher embryo survival and pregnancy rates with vitrification than after slow freeze Particularly in vitro and biopsied embryos benefit from this cryopreservation method Media should not be pre-heated to more than 30°C prior to use – leave lid on in order to avoid evaporation
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts.
The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants)
Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second
See the detailed protocol on Vitrification and Warming of embryosDOWNLOAD